Acta Anatomica Sinica

›› 2009, Vol. 40 ›› Issue (6): 933-937.doi: 10.3969/j.issn.0529-1356.2009.06.017

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Expressions of hepatocyte nuclear factor -1α and hepatocyte nuclear factor -4α in human hepatocellular carcinoma

  

  1. 1.Department of Pathology, Weifang Medical College, Shandong Weifang 261042, China; 2. Department of Hepatobiliary Surgery, Weifang People’s Hospital, Shandong Weifang 261042, China; 3. Department of Anatomy, Weifang Medical College, Shandong Weifang 261042, China
  • Received:2008-10-06 Revised:2008-11-27 Online:2009-12-06
  • Contact: JIANG Ji-ying

Abstract: Objective To investigate the expression of hepatocyte nuclear factor-1α (HNF-1α) and hepatocyte nuclear factor-4α (HNF-4α) in human hepatocellular carcinoma (HCC) and explore the function of HNF-1α and HNF-4α during HCC carcinogenesis and development. Methods Twenty-six specimens of hepatocellular carcinoma were collected. The expressions of HNF-1α and HNF-4α in HCC tissues and adjacent non-cancerous tissues were detected by reverse transcription polymerase chain reaction (RT-PCR) and immunohistochemistry staining. Results The mRNA levels of HNF-1α and HNF-4α were significantly lower in HCC tissues than that in adjacent non-cancerous tissues (0.818±0.371 vs. 0.383±0.102 for HNF-1α, P0.05; 0.846±0.384 vs. 0.397±0.105 for HNF-4α, P0.05）.The positive rates of HNF-1α and HNF-4α protein were significantly lower in HCC tissues than in adjacent non-c

Key words: HNF-1α, HNF-4α, Hepatocellular carcinoma, RT-PCR, Immunohistochemistry, Human

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